Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides
long, usually with a two-base overhang on the 3' end. Dicer contains
two RNase III domains and one PAZ domain; the distance between these two
regions of the molecule is determined by the length and angle of the
connector helix and determines the length of the siRNAs it produces.Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand.The human version of this gene is DICER1. The
determination of Dicer expression is of critical importance in the
diagnosis of human cancer. Mouse monoclonal 4A6 is of particular utility for IHC on FFPE sections.
Form : Hybridoma supernatant in high stability buffer Applications IHC , IHC (FFPE), ELISA, IP, WB , CLIP, CHIP Sensitivity WB (with ECL): 10-50ng of purified Dicer protein Working Conditions WB (with ECL): 1:100-500 dilution (incubate for one hour at room temperature) IHC :1/200-500 For best results with other assays ,please determine optimal working dilution by titration test Storage Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods.
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