Anti-GST TAG Monoclonal CG045 (clone GST.B6)
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This is a well characterized and widely used monoclonal against the GST tag protein.
Description Anti-GST Tag Monoclonal Antibody
Host Species Mouse
Isotype IgG2a
Immunogen GST protein
Specificity Recognizes native and denatured forms of purified GST or GST fusion proteins
Purification Protein A affinity chromatography from mouse ascites fluid
Applications Dot, ELISA, IP, IS, WB
Sensitivity WB (with ECL): 10-50ng of purified GST or GST fusion proteins
Working Conditions WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles
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| Fig. 1:5000 dilution probed against BL-21 bacteria lysate expressing the GST protein |
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