| Anti-His Tag (Clone His.H8 ) Catalogue number CG0081 |
Description Anti-His Tag (HIS.H8 / EH158) Monoclonal Antibody
Clonality HIS.H8 / EH158
Host Species Mouse
Isotype IgG2b
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Purification Protein A affinity chromatography from mouse ascites fluid
Applications Dot, ELISA, IP, IS, WB
Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins
Form 1mg/mL in 10mM PBS, pH 7.2
Working Conditions WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles
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