| Anti-DYKDDDDK (FLAG) Tag - Cat # CG0093 |
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Mouse Monoclonal FG4R - IgG2b
Immunogen
DYKDDDDK
(FLAG) synthetic peptide conjugated to KLH
Specificity
Recognizes
the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Purification
Protein
A affinity chromatography from mouse ascites fluid
Application
Dot,
ELISA, IP, IS, WB
Sensitivity
WB
(with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins
Form
1mg/mL
in 10mM PBS, pH 7.2, with 0.05% NaN3
Working Condition
WB
(with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS:
1:500-2000 dilution
For
best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine
optimal working dilution by titration test
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| Western Blot of Anti-FLAG tag |
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