CLONEGENE

Clonality | Host Species | Isotype

HA.C5 / C10 | Mouse | IgG3

 Immunogen

YPYDVPDYA (HA) synthetic peptide conjugated to KLH

 Specificity

Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins

 Purification

Protein A affinity chromatography from mouse ascites fluid

 Application

Dot. ELISA, IP, IS, WB

 Sensitivity

WB (with ECL): 1-5ng of HA-tagged fusion proteins

 Form

1mg/mL in 10mM PBS, pH 7.2, with 0.05% NaN₃

 Working Condition

WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS: 1:500-2000 dilution

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test