|
Clonality | Host Species | Isotype
Myc.A7
/ M19 | Mouse | IgG1
Immunogen
EQKLISEEDL
(Myc) synthetic peptide conjugated to KLH
Specificity
Recognizes
the N-terminal or C-terminal Myc-tagged fusion proteins
Purification
Protein
A affinity chromatography from mouse ascites fluid
Application
Dot,
ELISA, IP, IS, WB
Sensitivity
WB
(with ECL): 10-50ng of Myc-tagged fusion proteins
Form
1mg/mL
in 10mM PBS, pH 7.2, with 0.05% NaN3
Working Condition
WB
(with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS:
1:500-2000 dilution
For
best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine
optimal working dilution by titration test
|
 |
| Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2) |
|
|