| Anti-Dicer Monoclonal Antibody 4A6 Cat# CG016 |
Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides
long, usually with a two-base overhang on the 3' end. Dicer contains
two RNase III domains and one PAZ domain; the distance between these two
regions of the molecule is determined by the length and angle of the
connector helix and determines the length of the siRNAs it produces.Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand.The human version of this gene is DICER1. The
determination of Dicer expression is of critical importance in the
diagnosis of human cancer. Clonegene offers two monoclonal antibodies
CG006 and CG016 which have both been characterized by siRNA validation.
CG016 is of particular utility for IHC on paraffin embeded sections.
For further information go to the user blog site
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| Dicer Expression in Ovary via 4A6 |
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Description Anti-Dicer Monoclonal Antibody
Host Species Mouse
Isotype IgG1
Immunogen Dicer peptide
Form- Hybridoma supernatant in high stability buffer
Applications Dot, ELISA, IP, IS, WB
Sensitivity WB (with ECL): 10-50ng of purified Dicer protein
Working Conditions WB (with ECL): 1:100-500 dilution (incubate for one hour at room temperature)
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods.
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