| Anti-Dicer Mouse Monoclonal 13D6 |
Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides
long, usually with a two-base overhang on the 3' end. Dicer contains
two RNase III domains and one PAZ domain; the distance between these two
regions of the molecule is determined by the length and angle of the
connector helix and determines the length of the siRNAs it produces.Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand. The human version of this gene is DICER1. Clonegene provides two mouse monoclonal antibodies against Dicer ,13D6 and 4A6, which have both been characterized by
siRNA validation. 13D6 and 4A6 monoclonal antibodies have been used in published studies on analysis of Dicer expression in FFPE sections from human tumors. There are several published studies indicating the prognostic potential of Dicer expression. These antibodies have also been extensively used in western blot and DNA and RNA immunoprecipitation studies.
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| siRNA VALIDATION of 13D6 |
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Raised in Mouse
Monoclonal: Clone number 13D6
Isotype: IgG1
Purity : Protein G purified
Storage buffer: Proprietary high stability buffer
Shipped at ambient temperature, advise long term storage -20.
Works for WB, IHC, IP , IF ,CHIP, CLIP, IHC on FFPE
Reacts with human, mouse, rat and lizard Dicer
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Having trouble using mouse monoclonal on mouse tissues ? It is a problem, since if you are using an anti-mouse IgG secondary, endogenous mouse IgG in the tissue will yield a troublesome background. Sidestep this problem by using a biotinylated primary , skip the secondary and just directly use Avidin /Biotin Peroxidase. Try our biotinylated anti-Dicer 13D6 monoclonal. Fluorescent conjugates are also available on enquiry.
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Need to check your immunoperoxidase staining ? Real staining will be displaced by co-incubation with the synthetic peptide . Dicer 13D6 immunogenic peptide is available here.
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