CLONEGENE

Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides long, usually with a two-base overhang on the 3' end. Dicer contains two RNase III domains and one PAZ domain; the distance between these two regions of the molecule is determined by the length and angle of the connector helix and determines the length of the siRNAs it produces. Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand. The human version of this gene is DICER1.  In recent work, CLONEGENE has developed a rabbit antibody (13D6R)  against a highly conserved peptide in human Dicer. This Dicer antibody reacts with human, mouse, rat, cow, dog and lizard Dicer . The sequence of the peptide is confidential but we are happy to advise you if  this antibody will work on Dicer from your species of interest.   Working conditions are 1/5,000 for IHC and 1/2000 for western blot.  13D6R was generated  against the same  synthetic peptide that was used to generate our anti-Dicer 13D6 and  anti-Dicer 4A6 mouse monoclonals. Indeed, 13D6R anti-Dicer antibody   is superior to 4A6 in IHC on archival paraffin sections (see image of 13D6R on  FFPE  lung tumor section ). 13D6R is provided as an  affinity-purified rabbit IgG (1mg/ml).