CLONEGENE

HuD otherwise known as ELAV-like protein 4 is a protein that in humans is encoded by the ELAVL4 gene.[1][2]
The HuD/ELAVL4 protein is an RNA-binding protein.[3]
HuD is expressed only in neurons and it binds to AU-rich element-containing mRNAs. As a result of this interaction the half-life of the transcript is increased. HuD is important in neurons during brain development and plasticity.[4][5]
Monoclonal  15A9  was designed by CLONEGENE and was generated against a unique HuD peptide and does not react with HuR, HuC, or Hel-N1.  Monoclonal 15A9  works well in western blot and IHC  and provides an excellent marker for neuronal cells.  but was specifically selected becasue of it's abiity to supershift HuD/mRNA ribonucleoprotein complexes. Thus, it is the ideal reagent for CHIP or RIP studies to identify novel mRNA targets in neuronal cells.
 

References

1 Muresu R, Baldini A, Gress T, Posner JB, Furneaux HM, Siniscalco M (Dec 1993). "Mapping of the gene coding for a paraneoplastic encephalomyelitis antigen (HuD) to human chromosome site 1p34". Cytogenet Cell Genet 65 (3): 177–8. doi:10.1159/000133626. PMID 8222755.
2"Entrez Gene: ELAVL4 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)".
3 Szabo A, Dalmau J, Manley G, et al. (1991). "HuD, a paraneoplastic encephalomyelitis antigen, contains RNA-binding domains and is homologous to Elav and Sex-lethal.". Cell 67 (2): 325–33. doi:10.1016/0092-8674(91)90184-Z. PMID 1655278.
4 Nora Perrone-Bizzozero and Federico Bolognani (2002). "Role of HuD and other RNA-binding proteins in neural development and plasticity". J Neurosci Res 68 (2): 121–126. doi:10.1002/jnr.10175. PMID 11948657.
5 Federico Bolognani and Nora Perrone-Bizzozero (2008). "RNA-protein interactions and control of mRNA stability in neurons". J Neurosci Res 86 (3): 481–489. doi:10.1002/jnr.21473. PMID 17853436.
6 Saito, Kuniaki; Fujiwara Toshinobu, Katahira Jun, Inoue Kunio, Sakamoto Hiroshi (Aug. 2004). "TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD". Biochem. Biophys. Res. Commun. (United States) 321 (2): 291–7. doi:10.1016/j.bbrc.2004.06.140. ISSN 0006-291X. PMID 15358174.

Mouse monoclonal
Clone number  15A9
Isotype   IgG2b
Preparation - Purified Antibody
Transport  -  Shipped at ambient temperature
Storage -  On arrival aliquot and store at -20,  a working aliquot is stable at room temperature for weeks.
Works for WB, IHC, IP, CHIP, CLIP, RIP , IHC on FFPE sections.
Reacts with human, mouse, rat, xenopus and  zebrafish HuD.