CG 007 - Loading Control - Anti-GAPDH
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| Description |
Anti-GAPDH Loading Control Monoclonal Antibody |
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| Host Species |
Mouse |
| Isotype |
IgG1 |
| Immunogen |
Recombinant GAPDH |
| Specificity |
Recognizes native and denatured forms of GAPDH (∼37kDa) |
| Cross Reactivity |
GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster
GAPDH from other species may also be detectable |
| Purification |
Protein A affinity chromatography from mouse ascites fluid |
| Applications |
Dot, ELISA, IS, WB (see notations) |
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| Working Conditions |
WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA,
etc), please determine optimal working dilution by titration test |
| Storage |
Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles |
| Images |
 
Fig. LEFT: 1:2000 (0.5μg/mL) Ab
dilution used in WB of 5μg/lane tissue lysates from human (1), mouse
(2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3) |
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