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Anti- DDDDK

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Anti-PD1 Monoclonal J110

Anti-PD-L1 Monoclonal

Anti TIGIT

Anti-EGFR mab 225

Gene Regulation

Anti-Dicer MAb 4A6

Anti-Dicer Mab 13D6

Anti-Dicer 13D6R

HuR Antibody mab 19F12

HuR antibody 4C8

HuD RNA binding Protein

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ORDER INFO

Distributors

CG031 Anti-Dicer Affinity Purified Rabbit Antibody 13D6R
Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. Being part of the RNase III family, Dicer cleaves double-stranded RNA (dsRNA) and pre-microRNA (pre-miRNA) into short double-stranded RNA fragments called small interfering RNA and microRNA, respectively. These fragments are approximately 20-25 base pairs long with a two-base overhang on the 3' end. Dicer facilitates the activation of the RNA-induced silencing complex (RISC), which is essential for RNA interference. RISC has a catalytic component argonaute, which is an endonuclease capable of degrading messenger RNA (mRNA).

Dicer can be used to identify whether tumors are present within the body based on the expression level of the enzyme. A study showed that many patients that had cancer had decreased expression levels of Dicer. The same study showed that lower Dicer expression correlated with lower patient survival length.[11] Along with being a diagnostic tool, Dicer can be used for treating patients by injecting foreign siRNA intravenously to cause gene silencing.[22]

The siRNA was shown to be delivered in two ways in mammalian species such as mice. One way would be to directly inject into the system, which would not require Dicer function. Another way would be to introduce it by plasmids that encode for short hairpin RNA, which are cleaved by Dicer into siRNA.[23]

One of the advantages of using Dicer to produce siRNA therapeutically would be the specificity and diversity of targets it can affect compared to what is currently being used such as antibodies or small molecular inhibitors. In general, small molecular inhibitors are difficult in terms of specificity along with unendurable side effects. Antibodies are as specific as siRNA, but it is limited by only being able to be used against ligands or surface receptors. On the other hand, low efficiency of intracellular uptake is the main obstacle of injection of siRNA.[11] Injected SiRNA has poor stability in blood and causes stimulations of non-specific immunity.[24] Also, producing miRNA therapeutically lacks in specificity because only 6-8 nucleotide base pairing is required for miRNA to attach to mRNA.[25]




Product name   
13D6R

Description  
Affinity purified  rabbit peptide antibody against Dicer  
 
Tested applications 
WB,  IHC

Immunogen 
Dicer peptide 


Form 
Liquid

Storage instructions
 Heat stable , shipped at ambient  temp  Upon delivery aliquot and store in fridge , longterm storage at  -20°C.

Storage buffer 
PBS, pH 7.2

Concentration
 100 µl at 1.0  mg/ml

Purification notes
Affinity purified  

 

13D6R on breast tissue + indicates antigen depletion
 
 


REFERENCES 

Zhang B, Chen H, Zhang L, et al. A dosage-dependent pleiotropic role of Dicer in prostate cancer growth and metastasis. Oncogene. 2014;33(24):3099-3108. doi:10.1038/onc.2013.281.



Standard Size  
100ug 

100ug 13D6R

$360.00



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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" 


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