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Gene Regulation

Anti-Dicer MAb 4A6

Anti-Dicer Mab 13D6

Anti-Dicer 13D6R

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HuD RNA binding Protein

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ORDER INFO

Distributors

CG016 Anti-Dicer Monoclonal Antibody Clone 4A6
Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides long, usually with a two-base overhang on the 3' end. Dicer contains two RNase III domains and one PAZ domain; the distance between these two regions of the molecule is determined by the length and angle of the connector helix and determines the length of the siRNAs it produces.Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand.The human version of this gene is DICER1. The determination of Dicer expression is of  critical importance in the diagnosis of human cancer. Mouse monoclonal  4A6  is of particular  utility for IHC on FFPE  sections. 
NSCLC lung tumor with 4A6
 
 
Description    Anti-Dicer Monoclonal Antibody
Host Species   Mouse
Isotype    IgG1
Immunogen   Dicer peptide

Form : Hybridoma supernatant in high stability buffer     
Applications   IHC , IHC (FFPE), ELISA, IP,  WB , CLIP, CHIP 
Sensitivity    WB (with ECL): 10-50ng of purified Dicer protein
Working Conditions    WB (with ECL): 1:100-500 dilution (incubate for one hour at room temperature)
                                IHC :1/200-500
For best results with other assays ,please determine optimal working dilution by titration test
Storage    Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. 

100ul hybridoma sup 4A6
$360.00
 
 


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